Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HL-60 cells, mouse brain tissue, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IP | See 2 publications below |
Product Information
15006-1-AP targets ARIH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARIH2 fusion protein Ag6507 相同性解析による交差性が予測される生物種 |
| Full Name | ariadne homolog 2 (Drosophila) |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC000422 |
| Gene Symbol | ARIH2 |
| Gene ID (NCBI) | 10425 |
| RRID | AB_2290024 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95376 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| IF protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| IP protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol ARIH2 is essential for embryogenesis, and its hematopoietic deficiency causes lethal activation of the immune system. | ||
Oncogene The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia. | ||
Cell Death Dis ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.
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