Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, Calu-1 cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Product Information
10090-2-AP targets ARL2BP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0138 Product name: Recombinant human ARL2BP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-163 aa of BC003087 Sequence: MDALEGESFALSFSSASDAEFDAVVGYLEDIIMDDEFQLLQRNFMDKYYLEFEDTEENKLIYTPIFNEYISLVEKYIEEQLLQRIPEFNMAAFTTTLQHHKDEVAGDIFDMLLTFTDFLAFKEMFLDYRAEKEGRGLDLSSGLVVTSLCKSSSLPASQNNLRH 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 2 binding protein |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 19-21 kDa |
| GenBank accession number | BC003087 |
| Gene Symbol | ARL2BP |
| Gene ID (NCBI) | 23568 |
| RRID | AB_2058518 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2Y0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARL2BP antibody 10090-2-AP | Download protocol |
| IP protocol for ARL2BP antibody 10090-2-AP | Download protocol |
| WB protocol for ARL2BP antibody 10090-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP. | ||
Am J Hum Genet Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.
| ||
Int J Oncol BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation. | ||
Invest Ophthalmol Vis Sci Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis. | ||
Neoplasia BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.
| ||
PLoS One BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.
|