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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARL3 Monoclonal antibody

ARL3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5B12

Cat no : 68421-1-Ig

Synonyms

ADP ribosylation factor like 3, ARFL3, ARL3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ARL3 Monoclonal antibody (68421-1-Ig)

    WB analysis of HeLa using 68421-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit testis tissue using ARL3 Monoclonal antibody (68421-1-Ig)

    WB analysis of rabbit testis using 68421-1-Ig

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat retina tissue using ARL3 Monoclonal antibody (68421-1-Ig)

    WB analysis of rat retina using 68421-1-Ig

    rat retina tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL3 Monoclonal antibody (68421-1-Ig)

    IF Staining of hTERT-RPE1 using 68421-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL3 antibody (68421-1-Ig, Clone: 2E5B12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inHeLa cells, rabbit testis tissue, rat retina tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68421-1-Ig targets ARL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, rabbit samples.

Tested Reactivity Human, rat, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARL3 fusion protein Ag21387 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 3
Calculated molecular weight 20 kDa
Observed molecular weight 20-24 kDa
GenBank accession numberBC009841
Gene symbol ARL3
Gene ID (NCBI) 403
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

Protocols

Product Specific Protocols
WB protocol for ARL3 antibody 68421-1-IgDownload protocol
IF protocol for ARL3 antibody 68421-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols