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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARL4D Polyclonal antibody

ARL4D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15267-1-AP

Synonyms

ARF4L, ARL4D, ARL6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARL4D Polyclonal antibody (15267-1-AP)

    WB analysis using 15267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15267-1-AP (ARL4D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse retina tissue, Y79 cells, rat retina tiissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15267-1-AP targets ARL4D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL4D fusion protein Ag7369 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 4D
Calculated molecular weight 22 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC000043
Gene symbol ARL4D
Gene ID (NCBI) 379
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL4D (ADP-ribosylation factor-like protein 4D) is also named as ARF4L. ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. ARL4D interacts with the C-terminal pleckstrin homology (PH) and polybasic c domains of cytohesin-2/ARNO in a GTP-dependent manner. Localization of ARL4D at the plasma membrane is GTP- and N-terminal myristoylation-dependent. ARL4D(Q80L), a putative active form of ARL4D, induced accumulation of cytohesin-2/ARNO at the plasma membrane  (PMID: 17804820).

Protocols

Product Specific Protocols
WB protocol for ARL4D antibody 15267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols