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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARL4D Recombinant antibody

ARL4D Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241503B7

Cat no : 84163-3-RR

Synonyms

ARF4L, ARL6, ADP-ribosylation factor-like protein 4L, ADP-ribosylation factor-like protein 4D, 241503B7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARL4D Recombinant antibody (84163-3-RR)

    WB analysis using 84163-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84163-3-RR (ARL4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84163-3-RR

    Affinity and Kinetic Characterization of 84163-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84163-3-RR against Human ARL4D were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inY79 cells, mouse retina tissue, rat retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84163-3-RR is ARL4D Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARL4D fusion protein Ag7369 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 4D
Calculated molecular weight 22 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC000043
Gene symbol ARL4D
Gene ID (NCBI) 379
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL4D (ADP-ribosylation factor-like protein 4D) is also named as ARF4L. ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. ARL4D interacts with the C-terminal pleckstrin homology (PH) and polybasic c domains of cytohesin-2/ARNO in a GTP-dependent manner. Localization of ARL4D at the plasma membrane is GTP- and N-terminal myristoylation-dependent. ARL4D(Q80L), a putative active form of ARL4D, induced accumulation of cytohesin-2/ARNO at the plasma membrane  (PMID: 17804820).

Protocols

Product Specific Protocols
WB protocol for ARL4D antibody 84163-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols