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  • KD/KO Validated

ARL5B Polyclonal antibody

ARL5B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11694-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

ARL8, ADP-ribosylation factor-like protein 8, ADP-ribosylation factor-like protein 5B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inU2OS cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11694-1-AP targets ARL5B in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL5B fusion protein Ag2309 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 5B
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC024163
Gene Symbol ARL5B
Gene ID (NCBI) 221079
RRIDAB_2058835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KC2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL5B, also known as ADP ribosylation factor-like GTPase 5B, is a small G protein that is localized to the trans-Golgi network (TGN) in mammalian cells. It plays a significant role in the regulation of retrograde membrane transport from endosomes back to the TGN. This function is particularly important for the maintenance of cellular membrane traffic and the organization of the Golgi apparatus.

Protocols

Product Specific Protocols
WB protocol for ARL5B antibody 11694-1-APDownload protocol
IHC protocol for ARL5B antibody 11694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IP

J Biol Chem

Negative regulation of MDA5-dependent antiviral innate immune responses by Arl-like protein 5B.

Authors - Yuichi Kitai
  • KO Validated
mouseWB

J Cell Biochem

GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis.

Authors - Chun-Xiang Zhou
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