Validation Data Gallery
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Tested Applications
| Positive WB detected in | U2OS cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IP | See 1 publications below |
Product Information
11694-1-AP targets ARL5B in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARL5B fusion protein Ag2309 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 5B |
| Calculated molecular weight | 179 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC024163 |
| Gene Symbol | ARL5B |
| Gene ID (NCBI) | 221079 |
| RRID | AB_2058835 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL5B, also known as ADP ribosylation factor-like GTPase 5B, is a small G protein that is localized to the trans-Golgi network (TGN) in mammalian cells. It plays a significant role in the regulation of retrograde membrane transport from endosomes back to the TGN. This function is particularly important for the maintenance of cellular membrane traffic and the organization of the Golgi apparatus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL5B antibody 11694-1-AP | Download protocol |
| IHC protocol for ARL5B antibody 11694-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Negative regulation of MDA5-dependent antiviral innate immune responses by Arl-like protein 5B.
| ||
J Cell Biochem GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis. |