- Featured Product
- KD/KO Validated
ARL8A Polyclonal antibody
ARL8A Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 17060-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HepG2 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
17060-1-AP targets ARL8A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARL8A fusion protein Ag10788 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 8A |
| Calculated molecular weight | 186 aa, 21 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC015408 |
| Gene symbol | ARL8A |
| Gene ID (NCBI) | 127829 |
| RRID | AB_2058998 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A has a calculated molecular weight of 21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL8A antibody 17060-1-AP | Download protocol |
| IHC protocol for ARL8A antibody 17060-1-AP | Download protocol |
| IP protocol for ARL8A antibody 17060-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy | ||
Nat Commun RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. | ||
Curr Biol ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1. | ||
J Cell Biol Specific KIF1A-adaptor interactions control selective cargo recognition.
| ||
J Cell Sci The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.
| ||
