Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, human brain tissue, mouse brain tissue, NIH/3T3 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 11 publications below |
WB | See 18 publications below |
IHC | See 1 publications below |
IF | See 9 publications below |
CoIP | See 1 publications below |
Product Information
13049-1-AP targets ARL8B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, monkey, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ARL8B fusion protein Ag3711 相同性解析による交差性が予測される生物種 |
Full Name | ADP-ribosylation factor-like 8B |
Calculated molecular weight | 186 aa, 22 kDa |
Observed molecular weight | 22 kDa |
GenBank accession number | BC013131 |
Gene Symbol | ARL8B |
Gene ID (NCBI) | 55207 |
RRID | AB_2059000 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NVJ2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.
Protocols
Product Specific Protocols | |
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WB protocol for ARL8B antibody 13049-1-AP | Download protocol |
IHC protocol for ARL8B antibody 13049-1-AP | Download protocol |
IF protocol for ARL8B antibody 13049-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy | ||
Sci Adv The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.
| ||
Sci Adv Endosomal sorting drives the formation of axonal prion protein endoggresomes.
| ||
Nat Commun RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. | ||
J Cell Biol The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration. |