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  • KD/KO Validated

ARL8B Polyclonal antibody

ARL8B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13049-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

GIE1, EC:3.6.5.2, ARL10C, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, mouse brain tissue, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13049-1-AP targets ARL8B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL8B fusion protein Ag3711 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 8B
Calculated molecular weight 186 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC013131
Gene Symbol ARL8B
Gene ID (NCBI) 55207
RRIDAB_2059000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Protocols

Product Specific Protocols
WB protocol for ARL8B antibody 13049-1-APDownload protocol
IHC protocol for ARL8B antibody 13049-1-APDownload protocol
IF protocol for ARL8B antibody 13049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Lysosomal positioning coordinates cellular nutrient responses.

Authors - Korolchuk Viktor I VI
  • KD Validated
humanWB

Nat Commun

DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy

Authors - Rahul Kumar
monkeyWB

Sci Adv

The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.

Authors - Meng Lu
  • KD Validated
mouseWB,IF

Sci Adv

Endosomal sorting drives the formation of axonal prion protein endoggresomes.

Authors - Romain Chassefeyre
  • KO Validated
humanWB

Nat Commun

RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.

Authors - Tal Keren-Kaplan
mouseWB

J Cell Biol

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.

Authors - Natalia Schiefermeier
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