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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis using 67891-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of LNCaP cells using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis of LNCaP using 67891-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis of Jurkat using 67891-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis of Jurkat using 67891-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis of K-562 using 67891-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis of HSC-T6 using 67891-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis of NIH/3T3 using 67891-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using ARL8B Monoclonal antibody (67891-1-Ig)

    WB analysis of pig brain using 67891-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ARL8B Monoclonal antibody (67891-1-Ig)

    IF Staining of A549 using 67891-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8B antibody (67891-1-Ig, Clone: 2G10E2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

ARL8B Monoclonal antibody

ARL8B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67891-1-Ig
Clone No.2G10E2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

ARL10C, ARL8B, FLJ10702, Gie1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells, pig brain tissue, rat brain tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67891-1-Ig targets ARL8B in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3711

Product name: Recombinant human ARL8B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC013131

Sequence: MLALISRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIWDIGGQPRFRSMWERYCRGVNAIVYMIDAADREKIEASRNELHNLLDKPQLQGIPVLVLGNKRDLPNALDEKQLIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 8B
Calculated molecular weight 186 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC013131
Gene Symbol ARL8B
Gene ID (NCBI) 55207
RRIDAB_2918647
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NVJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Protocols

Product Specific Protocols
WB protocol for ARL8B antibody 67891-1-IgDownload protocol
IF protocol for ARL8B antibody 67891-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols