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  • Featured Product
  • KD/KO Validated

ARMC10 Polyclonal antibody

ARMC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20506-1-AP

Synonyms

armadillo repeat containing 10, ARMC10, PNAS 112, SVH

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using ARMC10 Polyclonal antibody (20506-1-AP)

    WB analysis of A431 using 20506-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ARMC10 Polyclonal antibody (20506-1-AP)

    WB analysis of HEK-293 using 20506-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ARMC10 Polyclonal antibody (20506-1-AP)

    WB analysis of HepG2 using 20506-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARMC10 Polyclonal antibody (20506-1-AP)

    WB analysis of mouse brain using 20506-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ARMC10 Polyclonal antibody (20506-1-AP)

    WB analysis of mouse liver using 20506-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using ARMC10 Polyclonal antibody (20506-1-AP)

    IP experiment of A431 using 20506-1-AP

    IP result of anti-ARMC10 (IP:20506-1-AP, 4ug; Detection:20506-1-AP 1:500) with A431 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARMC10 Polyclonal antibody (20506-1-AP)

    IHC staining of human placenta using 20506-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARMC10 Polyclonal antibody (20506-1-AP)

    IF Staining of HepG2 using 20506-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARMC10 Polyclonal antibody (20506-1-AP)

    IF Staining of A431 using 20506-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HepG2 cells, mouse brain tissue, mouse liver tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20506-1-AP targets ARMC10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARMC10 fusion protein Ag14313 相同性解析による交差性が予測される生物種
Full Name armadillo repeat containing 10
Calculated molecular weight 343 aa, 38 kDa
Observed molecular weight 31-37 kDa
GenBank accession numberBC003586
Gene symbol ARMC10
Gene ID (NCBI) 83787
RRIDAB_10696176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARMC10 antibody 20506-1-APDownload protocol
IHC protocol for ARMC10 antibody 20506-1-APDownload protocol
IF protocol for ARMC10 antibody 20506-1-APDownload protocol
IP protocol for ARMC10 antibody 20506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Death Dis

The Armc10/SVH gene: genome context, regulation of mitochondrial dynamics and protection against Aβ-induced mitochondrial fragmentation.

Authors - R Serrat
View Article
humanWB

Heliyon

Prognostic value and immune infiltration of ARMC10 in pancreatic adenocarcinoma via integrated bioinformatics analyses

Authors - Tian-Hao Li
  • KD Validated
View Article
humanWB,IF

J Cell Mol Med

ARMC10 regulates mitochondrial dynamics and affects mitochondrial function via the Wnt/β-catenin signalling pathway involved in ischaemic stroke

Authors - Yanyang Huang
  • KD Validated
View Article