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ARNT/HIF-1 beta Polyclonal antibody

Name: ARNT/HIF-1 beta Polyclonal antibody
Brand: Proteintech
SKU: 14105-1-AP

ARNT/HIF-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14105-1-AP

Synonyms

ARNT, HIF1B, HIF1 beta, HIF 1 beta, Class E basic helix-loop-helix protein 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 14105-1-AP
WB result of ARNT/HIF-1 beta antibody (14105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT,HIF1B transfected HeLa cells.
WB analysis of HepG2 using 14105-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 14105-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14105-1-AP
IP result of anti-ARNT/HIF-1 beta (IP:14105-1-AP, 4ug; Detection:14105-1-AP 1:1000) with HepG2 cells lysate 3600ug.
IHC staining of mouse embryo using 14105-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse embryo using 14105-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse embryo using 14105-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14105-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 14105-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	mouse embryo tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 16 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 2 publications below

Product Information

14105-1-AP targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARNT/HIF-1 beta fusion protein Ag5238 相同性解析による交差性が予測される生物種
Full Name	aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight	416 aa, 46 kDa, 87 kDa
Observed molecular weight	86 kDa
GenBank accession number	BC060838
Gene symbol	ARNT/HIF-1 beta
Gene ID (NCBI)	405
RRID	AB_10639523
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Protocols

Product Specific Protocols
WB protocol for ARNT/HIF-1 beta antibody 14105-1-AP	Download protocol
IHC protocol for ARNT/HIF-1 beta antibody 14105-1-AP	Download protocol
IF protocol for ARNT/HIF-1 beta antibody 14105-1-AP	Download protocol
IP protocol for ARNT/HIF-1 beta antibody 14105-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Hepatology PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability. Authors - Liu Yanfeng Y View Article
human	WB,IF	BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma Authors - Yizhi Zhan View Article
human	WB	Free Radic Biol Med Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation. Authors - Kyuhwa Seo View Article
mouse	WB	Cell Immunol Fine particulate matter (PM2.5) induces inhibitory memory alveolar macrophages through the AhR/IL-33 pathway Authors - Yanan Liu View Article
mouse	WB	Front Microbiol Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection. Authors - Zhixiao Sun View Article
mouse	WB	J Biomed Nanotechnol Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway. Authors - Zhongqi Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARNT/HIF-1 beta Polyclonal antibody

ARNT/HIF-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 14105-1-AP

WB analysis of HepG2 using 14105-1-AP

WB analysis using 14105-1-AP

IP experiment of HepG2 using 14105-1-AP

IHC staining of mouse embryo using 14105-1-AP

IHC staining of mouse embryo using 14105-1-AP

IHC staining of mouse embryo using 14105-1-AP

IF Staining of HepG2 using 14105-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability.

NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma

Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation.

Fine particulate matter (PM2.5) induces inhibitory memory alveolar macrophages through the AhR/IL-33 pathway

Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.

Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway.