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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Recombinant antibody (84650-4-RR)

    WB analysis using 84650-4-RR (same clone as 84650-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84650-4-RR (ARNT,HIF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARNT/HIF-1 beta Recombinant antibody (84650-4-RR)

    IF Staining of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARNT,HIF1B antibody (84650-4-RR, Clone: 242111G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ARNT/HIF-1 beta Recombinant antibody (84650-4-RR)

    FC experiment of HeLa using 84650-4-RR (same clone as 84650-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ARNT/HIF-1 beta Recombinant antibody (84650-4-RR, Clone:242111G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using ARNT/HIF-1 beta Recombinant antibody (84650-4-RR)

    FC experiment of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ARNT/HIF-1 beta Recombinant antibody (84650-4-RR, Clone:242111G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84650-4-RR

    Affinity and Kinetic Characterization of 84650-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84650-4-RR against Human ARNT/HIF-1 beta were performed. The affinity constant is 0.109 nM.

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ARNT/HIF-1 beta Recombinant antibody, PBS Only

ARNT/HIF-1 beta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84650-4-PBS
Clone No.242111G2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84650-4-PBS targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARNT/HIF-1 beta fusion protein Ag5507 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP27540
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.