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  • Featured Product
  • KD/KO Validated

ARNT/HIF-1 beta Polyclonal antibody, PBS Only

ARNT/HIF-1 beta Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14105-1-PBS

Synonyms

ARNT, HIF1B, HIF1 beta, HIF 1 beta, Class E basic helix-loop-helix protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    WB analysis of HeLa using 14105-1-AP (same clone as 14105-1-PBS)

    WB result of ARNT/HIF-1 beta antibody (14105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT,HIF1B transfected HeLa cells. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    WB analysis of HepG2 using 14105-1-AP (same clone as 14105-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    WB analysis using 14105-1-AP (same clone as 14105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    IP experiment of HepG2 using 14105-1-AP (same clone as 14105-1-PBS)

    IP result of anti-ARNT/HIF-1 beta (IP:14105-1-AP, 4ug; Detection:14105-1-AP 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    IHC staining of mouse embryo using 14105-1-AP (same clone as 14105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    IHC staining of mouse embryo using 14105-1-AP (same clone as 14105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    IHC staining of mouse embryo using 14105-1-AP (same clone as 14105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARNT/HIF-1 beta Polyclonal antibody (14105-1-AP)

    IF Staining of HepG2 using 14105-1-AP (same clone as 14105-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14105-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14105-1-PBS targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARNT/HIF-1 beta fusion protein Ag5238 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC060838
Gene symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia