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- KD/KO Validated
ARNTL Polyclonal antibody
ARNTL Polyclonal Antibody for WB, IP, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, IHC, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 14268-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-1080 cells, 3T3-L1 cells, HeLa cells, MCF-7 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 7 publications below |
WB | See 39 publications below |
IHC | See 12 publications below |
IF | See 5 publications below |
CoIP | See 1 publications below |
ChIP | See 4 publications below |
Product Information
14268-1-AP targets ARNTL in WB, IP, IF, FC, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ARNTL fusion protein Ag5586 相同性解析による交差性が予測される生物種 |
Full Name | aryl hydrocarbon receptor nuclear translocator-like |
Calculated molecular weight | 69 kDa |
Observed molecular weight | 69-75 kDa |
GenBank accession number | BC041129 |
Gene symbol | ARNTL |
Gene ID (NCBI) | 406 |
RRID | AB_2878037 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ARNTL antibody 14268-1-AP | Download protocol |
IHC protocol for ARNTL antibody 14268-1-AP | Download protocol |
IP protocol for ARNTL antibody 14268-1-AP | Download protocol |
FC protocol for ARNTL antibody 14268-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
J Pineal Res Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors | ||
Sci Total Environ Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario | ||
Theranostics Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia. | ||
Environ Pollut Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction. |