ARPC2 Monoclonal antibody
ARPC2 Monoclonal Antibody for WB, IHC, ELISA, IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1D8E3
Cat no : 68145-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, human peripheral blood platelets, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68145-1-Ig targets ARPC2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
Tested Reactivity | Human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ARPC2 fusion protein Ag7082 相同性解析による交差性が予測される生物種 |
Full Name | actin related protein 2/3 complex, subunit 2, 34kDa |
Calculated molecular weight | 34 kDa |
Observed molecular weight | 34 kDa |
GenBank accession number | BC000590 |
Gene symbol | ARPC2 |
Gene ID (NCBI) | 10109 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ARPC2 antibody 68145-1-Ig | Download protocol |
IHC protocol for ARPC2 antibody 68145-1-Ig | Download protocol |
IF protocol for ARPC2 antibody 68145-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |