ARPC4 Polyclonal antibody

Name: ARPC4 Polyclonal antibody
Brand: Proteintech
SKU: 10930-1-AP

ARPC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10930-1-AP

Synonyms

ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of mouse brain using 10930-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10930-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of 4T1 using 10930-1-AP
4T1 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of human brain using 10930-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10930-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 10930-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 10930-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat lymph using 10930-1-AP
rat lymph tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10930-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 10930-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, mouse small intestine tissue, rat lymph tissue, 4T1 cells, human placenta tissue, human brain tissue, mouse cerebellum tissue, MCF-7 cells
Positive IHC detected in	human cervical cancer tissue, human testis tissue, human lung cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

10930-1-AP targets ARPC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARPC4 fusion protein Ag1366 相同性解析による交差性が予測される生物種
Full Name	actin related protein 2/3 complex, subunit 4, 20kDa
Calculated molecular weight	20 kDa
Observed molecular weight	19-25 kDa
GenBank accession number	BC012596
Gene symbol	ARPC4
Gene ID (NCBI)	10093
RRID	AB_2059951
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The ARP2/3 complex consists of seven subunits: two actin-related proteins (ARP2 and ARP3) and five ARP2/3 complex polypeptides (ARPC1B and ARPC2-5). ARPC4 is a core subunit of the actin-related protein (ARP2/3) complex, which catalyzes the formation of F-actin networks. ARPC4 is essential for the structural integrity of the ARP2/3 complex function. Mutations in ARPC4 cause Developmental delay, language impairment, and ocular abnormalities (DEVLO)(PMID: 35047857).

Protocols

Product Specific Protocols
WB protocol for ARPC4 antibody 10930-1-AP	Download protocol
IHC protocol for ARPC4 antibody 10930-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Life Sci Alliance A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells Authors - Sumana Sharma View Article
human	WB	Biochim Biophys Acta Proteins Proteom Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis. Authors - Guoxuan Qin View Article
human	WB	Drug Resist Updat Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane Authors - Xin Zhang View Article
mouse,bovine	WB,IF	Biomolecules Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model Authors - Bo Fang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARPC4 Polyclonal antibody

ARPC4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 10930-1-AP

WB analysis of MCF-7 using 10930-1-AP

WB analysis of 4T1 using 10930-1-AP

WB analysis of human brain using 10930-1-AP

WB analysis of human placenta using 10930-1-AP

WB analysis of mouse small intestine using 10930-1-AP

WB analysis of mouse cerebellum using 10930-1-AP

WB analysis of rat lymph using 10930-1-AP

WB analysis of human brain using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human cervical cancer using 10930-1-AP

IHC staining of human cervical cancer using 10930-1-AP

IHC staining of human urothelial carcinoma using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells

Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis.

Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane

Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model