ARPC5 Polyclonal antibody

Name: ARPC5 Polyclonal antibody
Brand: Proteintech
SKU: 16717-1-AP

ARPC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16717-1-AP

Synonyms

p16-ARC, p16 Arc, Arp2/3 complex 16 kDa subunit, ARC16, Actin-related protein 2/3 complex subunit 5

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of mouse brain using 16717-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 16717-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 16717-1-AP
Rat brain lysates were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 16717-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16717-1-AP (ARPC5 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse kidney using 16717-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 16717-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 16717-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 16717-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Neuro-2a using 16717-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARPC5 antibody (16717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16717-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue, mouse thymus tissue
Positive IHC detected in	mouse kidney tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Neuro-2a cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below

Product Information

16717-1-AP targets ARPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARPC5 fusion protein Ag10144 相同性解析による交差性が予測される生物種
Full Name	actin related protein 2/3 complex, subunit 5, 16kDa
Calculated molecular weight	154 aa, 17 kDa
Observed molecular weight	18-20 kDa
GenBank accession number	BC057237
Gene symbol	ARPC5
Gene ID (NCBI)	10092
RRID	AB_1850902
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ARPC5 antibody 16717-1-AP	Download protocol
IHC protocol for ARPC5 antibody 16717-1-AP	Download protocol
IF protocol for ARPC5 antibody 16717-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis Authors - Jiying Hou View Article
human	WB	J Neurosci The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse. Authors - Avanti Gokhale View Article
human	WB	Pigment Cell Melanoma Res YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5) Authors - Jason W. Lui View Article
mouse	WB	J Reprod Dev MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling Authors - Lanfang Ma View Article
human	IHC	BMC Cancer ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas Authors - Yue Ming View Article
human	WB	Heliyon Dapagliflozin impedes endothelial cell senescence by activating the SIRT1 signaling pathway in type 2 diabetes Authors - Shi Tai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARPC5 Polyclonal antibody

ARPC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 16717-1-AP

WB analysis of mouse thymus using 16717-1-AP

WB analysis using 16717-1-AP

IHC staining of human gliomas using 16717-1-AP

IHC staining of mouse kidney using 16717-1-AP

IHC staining of mouse kidney using 16717-1-AP

IHC staining of mouse kidney using 16717-1-AP

IHC staining of mouse kidney using 16717-1-AP

IF Staining of Neuro-2a using 16717-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis

The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.

YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5)

MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling

ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas

Dapagliflozin impedes endothelial cell senescence by activating the SIRT1 signaling pathway in type 2 diabetes