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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARPC5L Polyclonal antibody, PBS Only

ARPC5L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22025-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis using 22025-1-AP (same clone as 22025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis of NIH/3T3 using 22025-1-AP (same clone as 22025-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis of mouse lung using 22025-1-AP (same clone as 22025-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    WB analysis of human brain using 22025-1-AP (same clone as 22025-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IP experiment of mouse brain using 22025-1-AP (same clone as 22025-1-PBS)

    IP result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human cerebellum using 22025-1-AP (same clone as 22025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARPC5L Polyclonal antibody (22025-1-AP)

    IHC staining of human cerebellum using 22025-1-AP (same clone as 22025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ARPC5L Polyclonal antibody (22025-1-AP)

    IF Staining of MCF-7 using 22025-1-AP (same clone as 22025-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 22025-1-AP (ARPC5L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22025-1-PBS targets ARPC5L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPC5L fusion protein Ag16881 相同性解析による交差性が予測される生物種
Full Name actin related protein 2/3 complex, subunit 5-like
Calculated molecular weight 17 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC000798
Gene symbol ARPC5L
Gene ID (NCBI) 81873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARPC5L, also known as ARC16-2, belongs to the ARPC5 family. ARPC5L is involved in Arp2/3 complex-mediated actin nucleation and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC5L-containing Arp2/3 complexes are required for the peripheral positioning of nuclei in myofibers (PMID: 28892082). The molecular mass of ARPC5L is 17-20 kDa.