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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACTRT3/ARPM1 Polyclonal antibody

ACTRT3/ARPM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27580-1-AP

Synonyms

actin related protein M1, ARPM1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ACTRT3/ARPM1 Polyclonal antibody (27580-1-AP)

    WB analysis using 27580-1-AP

    various lysates were subjected to SDS PAGE followed by western blot with 27580-1-AP (ARPM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ACTRT3/ARPM1 Polyclonal antibody (27580-1-AP)

    IHC staining of human colon using 27580-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27580-1-AP (ARPM1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ACTRT3/ARPM1 Polyclonal antibody (27580-1-AP)

    IHC staining of human colon using 27580-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27580-1-AP (ARPM1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ACTRT3/ARPM1 Polyclonal antibody (27580-1-AP)

    IHC staining of human testis using 27580-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27580-1-AP (ARPM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ACTRT3/ARPM1 Polyclonal antibody (27580-1-AP)

    IF Staining of U2OS using 27580-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 27580-1-AP (ARPM1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inrat testis tissue, mouse testis tissue
Positive IHC detected inhuman colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27580-1-AP targets ACTRT3/ARPM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTRT3/ARPM1 fusion protein Ag25951 相同性解析による交差性が予測される生物種
Full Name actin related protein M1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC007289
Gene symbol ACTRT3/ARPM1
Gene ID (NCBI) 84517
RRIDAB_2880914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACTRT3/ARPM1 antibody 27580-1-APDownload protocol
IHC protocol for ACTRT3/ARPM1 antibody 27580-1-APDownload protocol
IF protocol for ACTRT3/ARPM1 antibody 27580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Loss of Profilin3 Impairs Spermiogenesis by Affecting Acrosome Biogenesis, Autophagy, Manchette Development and Mitochondrial Organization.

Authors - Naila Umer
View Article