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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARSE Polyclonal antibody

ARSE Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31522-1-AP

Synonyms

CDPXR, CDPX1, CDPX, ASE, Arylsulfatase E

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using ARSE Polyclonal antibody (31522-1-AP)

    IHC staining of mouse liver using 31522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31522-1-AP (ARSE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ARSE Polyclonal antibody (31522-1-AP)

    IHC staining of mouse liver using 31522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31522-1-AP (ARSE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARSE Polyclonal antibody (31522-1-AP)

    IF Staining of HepG2 using 31522-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARSE antibody (31522-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31522-1-AP targets ARSE in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARSE fusion protein Ag24371 相同性解析による交差性が予測される生物種
Full Name arylsulfatase E (chondrodysplasia punctata 1)
GenBank accession numberBC130438
Gene symbol ARSE
Gene ID (NCBI) 415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARSE (arylsulfatase E), is a member of the sulfatase family. This enzyme is glycosylated post-translationally. Sulfatases, including ARSE, are essential for the correct composition of the bone and cartilage matrix.

Protocols

Product Specific Protocols
IHC protocol for ARSE antibody 31522-1-APDownload protocol
IF protocol for ARSE antibody 31522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols