ART1 Monoclonal antibody
ART1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D10A8
Cat no : 66958-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | EC109 cells, HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
66958-1-Ig targets ART1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ART1 fusion protein Ag28269 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosyltransferase 1 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 34-36 kDa |
| GenBank accession number | NM_004314 |
| Gene symbol | ART1 |
| Gene ID (NCBI) | 417 |
| RRID | AB_2882281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ART1 antibody 66958-1-Ig | Download protocol |
| IHC protocol for ART1 antibody 66958-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Med Oncol Identification of the role of mono-ADP-ribosylation in colorectal cancer by integrated transcriptome analysis. | ||
BMC Cancer ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells
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