ASAH1 Polyclonal antibody

ASAH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11274-1-AP

Synonyms

EC:3.5.1.-, ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase


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Tested Applications

Positive WB detected inSH-SY5Y cells, SW480 cells, T-47D cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11274-1-AP targets ASAH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASAH1 fusion protein Ag1799 相同性解析による交差性が予測される生物種
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Calculated molecular weight 44 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC016828
Gene symbol ASAH1
Gene ID (NCBI) 427
RRIDAB_2058719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). The predicted molecular weight of ASAH1 is 45 kDa. ASAH1 is a glycoprotein processed from a 55-60 kDa precursor via autoproteolytic cleavage into a mature heterodimeric enzyme formed by an α-subunit (13 kDa) and a β-subunit (37-40 kDa) (PMID: 22261821).

Protocols

Product Specific Protocols
WB protocol for ASAH1 antibody 11274-1-APDownload protocol
IHC protocol for ASAH1 antibody 11274-1-APDownload protocol
IF protocol for ASAH1 antibody 11274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.

Authors - Judith Lang
human,mouseWB

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang
humanWB

Hum Mol Genet

Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.

Authors - Myung Jong Kim
mouseIF

BMC Genomics

Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment

Authors - Yuanmin Jia
mouseWB

Int J Mol Sci

Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.

Authors - Yu-Ting Su
humanWB

J Dermatol Sci

Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.

Authors - Sawada Eri E