Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat cerebellum tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
67092-1-Ig targets ASAH1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ASAH1 fusion protein Ag28645 相同性解析による交差性が予測される生物種 |
| Full Name | N-acylsphingosine amidohydrolase (acid ceramidase) 1 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC016828 |
| Gene Symbol | ASAH1 |
| Gene ID (NCBI) | 427 |
| RRID | AB_2882397 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13510 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASAH1 antibody 67092-1-Ig | Download protocol |
| IHC protocol for ASAH1 antibody 67092-1-Ig | Download protocol |
| IF protocol for ASAH1 antibody 67092-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |