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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASAP3 Polyclonal antibody (14099-1-AP)

    WB analysis using 14099-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14099-1-AP (ASAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASAP3 Polyclonal antibody (14099-1-AP)

    IF Staining of HepG2 using 14099-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASAP3 antibody (14099-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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ASAP3 Polyclonal antibody

ASAP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14099-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Development and differentiation-enhancing factor-like 1, DDEFL1, CENTB6, ASAP 3, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14099-1-AP targets ASAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASAP3 fusion protein Ag5231 相同性解析による交差性が予測される生物種
Full Name ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Calculated molecular weight 903 aa, 99 kDa
Observed molecular weight100 kDa
GenBank accession numberBC060786
Gene Symbol ASAP3
Gene ID (NCBI) 55616
RRIDAB_2059021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling.

Protocols

Product Specific Protocols
WB protocol for ASAP3 antibody 14099-1-APDownload protocol
IHC protocol for ASAP3 antibody 14099-1-APDownload protocol
IF protocol for ASAP3 antibody 14099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Aging (Albany NY)

Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.

Authors - Jiaming Fan
View Article