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Target

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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

ASB6 Polyclonal antibody

ASB6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21449-1-AP

Synonyms

ASB 6, ASB6

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ASB6 Polyclonal antibody (21449-1-AP)

    WB analysis of L02 using 21449-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21449-1-AP (ASB6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ASB6 Polyclonal antibody (21449-1-AP)

    WB analysis of L02 using 21449-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21449-1-AP (ASB6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ASB6 Polyclonal antibody (21449-1-AP)

    WB analysis of HEK-293 using 21449-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21449-1-AP (ASB6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inL02 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21449-1-AP targets ASB6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASB6 fusion protein Ag13863 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and SOCS box-containing 6
Calculated molecular weight 421 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001719
Gene symbol ASB6
Gene ID (NCBI) 140459
RRIDAB_2878860
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASB6 antibody 21449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

CUL5-ASB6 Complex Promotes p62/SQSTM1 Ubiquitination and Degradation to Regulate Cell Proliferation and Autophagy.

Authors - Liyan Gong
View Article
humanWB

J Cancer

ASB6 as an Independent Prognostic Biomarker for Colorectal Cancer Progression Involves Lymphatic Invasion and Immune Infiltration

Authors - Qingyong Hu
  • KO Validated
View Article