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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASB8 Polyclonal antibody

ASB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11735-1-AP

Synonyms

PP14212, ASB-8, ASB 8

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ASB8 Polyclonal antibody (11735-1-AP)

    WB analysis of human heart using 11735-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11735-1-AP (ASB8 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ASB8 Polyclonal antibody (11735-1-AP)

    WB analysis of human heart using 11735-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11735-1-AP (ASB8 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ASB8 Polyclonal antibody (11735-1-AP)

    IHC staining of human colon cancer using 11735-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11735-1-AP (ASB8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASB8 Polyclonal antibody (11735-1-AP)

    IF Staining of HepG2 using 11735-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11735-1-AP (ASB8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11735-1-AP targets ASB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASB8 fusion protein Ag2361 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and SOCS box-containing 8
Calculated molecular weight 288 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001321
Gene symbol ASB8
Gene ID (NCBI) 140461
RRIDAB_2227489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASB8 antibody 11735-1-APDownload protocol
IHC protocol for ASB8 antibody 11735-1-APDownload protocol
IF protocol for ASB8 antibody 11735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols