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  • Featured Product
  • KD/KO Validated

ASC/TMS1 Polyclonal antibody, PBS Only

ASC/TMS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10500-1-PBS

Synonyms

PYCARD, hASC, Caspase recruitment domain-containing protein 5, CARD5, ASC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    WB analysis using 10500-1-AP (same clone as 10500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    WB analysis using 10500-1-AP (same clone as 10500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    WB analysis using 10500-1-AP (same clone as 10500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    WB analysis of A549 using 10500-1-AP (same clone as 10500-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    WB analysis of Raji using 10500-1-AP (same clone as 10500-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10500-1-AP (TMS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    IP experiment of HL-60 using 10500-1-AP (same clone as 10500-1-PBS)

    IP result of anti-ASC/TMS1 (IP:10500-1-AP, 4ug; Detection:10500-1-AP 1:8000) with HL-60 cells lysate 1720 ug. This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    IHC staining of human lung cancer using 10500-1-AP (same clone as 10500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    IHC staining of human lung cancer using 10500-1-AP (same clone as 10500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (ASC/TMS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    IHC staining of human lung cancer using 10500-1-AP (same clone as 10500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10500-1-AP (TMS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ASC/TMS1 Polyclonal antibody (10500-1-AP)

    IF Staining of MCF-7 using 10500-1-AP (same clone as 10500-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 10500-1-AP (TMS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10500-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10500-1-PBS targets ASC/TMS1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASC/TMS1 fusion protein Ag0774 相同性解析による交差性が予測される生物種
Full Name PYD and CARD domain containing
Calculated molecular weight 25 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC004470
Gene symbol ASC/TMS1
Gene ID (NCBI) 29108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.