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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASCC3 Recombinant antibody (85130-2-RR)

    WB analysis using 85130-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85130-2-RR (ASCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASCC3 Recombinant antibody (85130-2-RR)

    IF Staining of HeLa using 85130-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASCC3 antibody (85130-2-RR, Clone: 242755B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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ASCC3 Recombinant antibody

ASCC3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85130-2-RR
Clone No.242755B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

242755B4, Activating signal cointegrator 1 complex subunit 3, ASC-1 complex subunit p200, ASC1p200, EC:3.6.4.12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293t cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85130-2-RR targets ASCC3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASCC3 fusion protein Ag11848 相同性解析による交差性が予測される生物種
Full Name activating signal cointegrator 1 complex subunit 3
Calculated molecular weight 111 aa, 13 kDa, 251 kDa
Observed molecular weight245 kDa
GenBank accession numberBC050681
Gene Symbol ASCC3
Gene ID (NCBI) 10973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N3C0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASCC3 (Activating Signal Cointegrator 1 Complex Subunit 3) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, DNA repair, and ribosome quality control. It is a member of the ASCC (Activating Signal Cointegrator 1 Complex) family, which functions as a bridge between co-repressors and co-activators in transcriptional regulation.

Protocols

Product Specific Protocols
WB protocol for ASCC3 antibody 85130-2-RRDownload protocol
IF protocol for ASCC3 antibody 85130-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols