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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASCL1 Polyclonal antibody (23751-1-AP)

    WB analysis using 23751-1-AP (same clone as 23751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23751-1-AP (ASCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ASCL1 Polyclonal antibody (23751-1-AP)

    IHC staining of human brain using 23751-1-AP (same clone as 23751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23751-1-AP (ASCL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23751-1-PBS in a different storage buffer formulation.

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ASCL1 Polyclonal antibody, PBS Only

ASCL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 23751-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Achaete-scute homolog 1, ASH1, ASH-1, bHLHa46, Class A basic helix-loop-helix protein 46

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23751-1-PBS targets ASCL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASCL1 fusion protein Ag19360 相同性解析による交差性が予測される生物種
Full Name achaete-scute complex homolog 1 (Drosophila)
Calculated molecular weight 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC003134
Gene Symbol ASCL1
Gene ID (NCBI) 429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP50553
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.