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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASCL1 Polyclonal antibody

ASCL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31206-1-AP

Synonyms

Achaete scute homolog 1, ASCL1, ASH 1, ASH1, bHLHa46, HASH1, MASH1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASCL1 Polyclonal antibody (31206-1-AP)

    WB analysis using 31206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31206-1-AP (ASCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inK-562 cells, NCI-H1299 cells, U-251 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31206-1-AP targets ASCL1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASCL1 fusion protein Ag35185 相同性解析による交差性が予測される生物種
Full Name achaete-scute complex homolog 1 (Drosophila)
Calculated molecular weight 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC003134
Gene symbol ASCL1
Gene ID (NCBI) 429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASCL1 (achaete-scute family bHLH transcription factor 1), also known as ASH1. It is expected to be located in nucleus. Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach. The calculated molecular weight of ASCL1 is 25 kDa.

Protocols

Product Specific Protocols
WB protocol for ASCL1 antibody 31206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols