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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using ASCL2 Polyclonal antibody (21368-1-AP)

    WB analysis of Caco-2 using 21368-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 21368-1-AP (ASCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MKN-45 cells using ASCL2 Polyclonal antibody (21368-1-AP)

    WB analysis of MKN-45 using 21368-1-AP

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 21368-1-AP (ASCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ASCL2 Polyclonal antibody (21368-1-AP)

    IHC staining of human stomach cancer using 21368-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21368-1-AP (ASCL2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ASCL2 Polyclonal antibody

ASCL2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21368-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Class A basic helix-loop-helix protein 45, bHLHa45, ASH-2, ASH2, Achaete-scute homolog 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMKN-45 cells, Caco-2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21368-1-AP targets ASCL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASCL2 fusion protein Ag15839 相同性解析による交差性が予測される生物種
Full Name achaete-scute complex homolog 2 (Drosophila)
Calculated molecular weight 193 aa, 20 kDa
Observed molecular weight20 kDa
GenBank accession numberBC057801
Gene Symbol ASCL2
Gene ID (NCBI) 430
RRIDAB_2935452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Achaete-scute complex homolog 2 (Drosophila), also known as ASCL2, is an imprinted human gene. This gene is involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system, particularly important during implantation of the developing embryo, specifically in placental development and neuronal precursor determination. ASCL2 has been reported involved in tumor progression, being upregulated in colorectal tumors, and involvement in metastasis could be attributed to ASCL2-promoted cellular self-renewal as opposed to cellular differentiation (PMID: 30096149 16568095).

Protocols

Product Specific Protocols
WB protocol for ASCL2 antibody 21368-1-APDownload protocol
IHC protocol for ASCL2 antibody 21368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

tRNA-derived RNA fragment, tRF-18-8R6546D2, promotes pancreatic adenocarcinoma progression by directly targeting ASCL2

Authors - Sihua Lan
View Article