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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    IP experiment of HeLa using 66671-1-Ig (same clone as 66671-1-PBS)

    IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    IHC staining of human gliomas using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    IHC staining of human gliomas using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASF/SF2 Monoclonal antibody (66671-1-Ig)

    IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

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ASF/SF2 Monoclonal antibody, PBS Only

ASF/SF2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66671-1-PBS
Clone No.1A6C5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66671-1-PBS targets ASF/SF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASF/SF2 fusion protein Ag3626 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 1
Calculated molecular weight 248 aa, 28 kDa
Observed molecular weight 32 kDa, 28 kDa, 22 kDa
GenBank accession numberBC010264
Gene Symbol ASF/SF2
Gene ID (NCBI) 6426
RRIDAB_2882025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ07955
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.