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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ASF1A Monoclonal antibody (66884-1-Ig)

    WB analysis of Jurkat using 66884-1-Ig (same clone as 66884-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ASF1A Monoclonal antibody (66884-1-Ig)

    WB analysis of HSC-T6 using 66884-1-Ig (same clone as 66884-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASF1A Monoclonal antibody (66884-1-Ig)

    IF Staining of HepG2 using 66884-1-Ig (same clone as 66884-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1A antibody (66884-1-Ig, Clone: 2D4A6 ) at dilution of 1:500 and sms3AF647-1, CL488-Phalloidin (green). This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

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ASF1A Monoclonal antibody, PBS Only

ASF1A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66884-1-PBS
Clone No.2D4A6

Host / Isotype

Mouse / IgG3

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CGI-98, CGI 98, CCG1-interacting factor A, CCG1 interacting factor A, Anti-silencing function protein 1 homolog A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66884-1-PBS targets ASF1A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ASF1A fusion protein Ag1233 相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight 21-23 kDa
GenBank accession numberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2882216
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y294
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.