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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASF1A/B Polyclonal antibody

ASF1A/B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11011-1-AP

Synonyms

ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, CIA-II, CIA II

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of mouse thymus using 11011-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of mouse thymus using 11011-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of HeLa using 11011-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of mouse thymus using 11011-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    IHC staining of human lymphoma using 11011-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    IHC staining of human lymphoma using 11011-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASF1A/B Polyclonal antibody (11011-1-AP)

    IF Staining of HeLa using 11011-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11011-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse thymus tissue, HeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11011-1-AP targets ASF1A/B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF1A/B fusion protein Ag1477 相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC010014
Gene symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2059670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.

Protocols

Product Specific Protocols
WB protocol for ASF1A/B antibody 11011-1-APDownload protocol
IHC protocol for ASF1A/B antibody 11011-1-APDownload protocol
IF protocol for ASF1A/B antibody 11011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols