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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASF1B-specific Polyclonal antibody (22258-1-AP)

    WB analysis using 22258-1-AP (same clone as 22258-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22258-1-AP (ASF1B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ASF1B-specific Polyclonal antibody (22258-1-AP)

    WB analysis of A431 using 22258-1-AP (same clone as 22258-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 22258-1-AP (ASF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1B-specific Polyclonal antibody (22258-1-AP)

    IHC staining of human lymphoma using 22258-1-AP (same clone as 22258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1B-specific Polyclonal antibody (22258-1-AP)

    IHC staining of human lymphoma using 22258-1-AP (same clone as 22258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1B-specific Polyclonal antibody (22258-1-AP)

    IHC staining of human lymphoma using 22258-1-AP (same clone as 22258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1B-specific Polyclonal antibody (22258-1-AP)

    IHC staining of human lymphoma using 22258-1-AP (same clone as 22258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASF1B-specific Polyclonal antibody (22258-1-AP)

    IF Staining of HeLa using 22258-1-AP (same clone as 22258-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22258-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASF1B-specific Polyclonal antibody (22258-1-AP)

    IF Staining of HeLa using 22258-1-AP (same clone as 22258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASF1B-specific antibody (22258-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ASF1B-specific Polyclonal antibody, PBS Only

ASF1B-specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22258-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ASF1B, ASF1A/B, CIA-II, CIA II, CCG1-interacting factor A-II

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22258-1-PBS targets ASF1B-specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF1B-specific fusion protein Ag17763 相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight 20-22 kDa
GenBank accession numberBC010014
Gene Symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2879051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody specifically reacts with the 19kd ASF1B protein.