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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASGR1 Recombinant antibody (83691-3-RR)

    WB analysis using 83691-3-RR (same clone as 83691-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83691-3-RR (ASGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00681-3

    Sandwich ELISA standard curve of MP00681-3

    Sandwich ELISA standard curve of MP00681-3, Human ASGR1 Recombinant Matched Antibody Pair - PBS only. 83691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0946. 83691-3-PBS was HRP conjugated as the detection antibody. Range: 7.8-1000 pg/mL.

  • Sample test of MP00681-3

    Sample test of MP00681-3

    The mean human ASGR1 concentration was determined to be 175.6 ng/mL in HepG2 cell extract based on a 3.7 mg/mL extract load.

  • Sample test of MP00681-3

    Sample test of MP00681-3

    Serum of sixteen individual healthy human donors was measured. The ASGR1 concentration of detected samples was determined to be 1,563.4 pg/mL with a range of 450.5-3,950.7 pg/mL.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ASGR1 Recombinant antibody (83691-3-RR)

    IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ASGR1 Recombinant antibody (83691-3-RR)

    IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using ASGR1 Recombinant antibody (83691-3-RR)

    IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using ASGR1 Recombinant antibody (83691-3-RR)

    IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00681-1

    Cytometric bead array standard curve of MP00681-1

    Cytometric bead array standard curve of MP00681-1, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-2-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00681-3

    Cytometric bead array standard curve of MP00681-3

    Cytometric bead array standard curve of MP00681-3, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-1-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83691-3-RR

    Affinity and Kinetic Characterization of 83691-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83691-3-RR against Human ASGR1 were performed. The affinity constant is 1.47 nM.

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ASGR1 Recombinant antibody, PBS Only (Detector)

ASGR1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83691-3-PBS
Clone No.240672C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83691-3-PBS targets ASGR1 as part of a matched antibody pair:

MP00681-1: 83691-2-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array)

MP00681-3: 83691-1-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name asialoglycoprotein receptor 1
Calculated molecular weight33 kDa
Observed molecular weight 42-46 kDa
GenBank accession numberNM_001671.4
Gene Symbol ASGR1
Gene ID (NCBI) 432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07306-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.