ASGR2 Polyclonal antibody
ASGR2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11501-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human liver tissue |
Positive IP detected in | L02 cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
11501-2-AP targets ASGR2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ASGR2 fusion protein Ag2037 相同性解析による交差性が予測される生物種 |
Full Name | asialoglycoprotein receptor 2 |
Calculated molecular weight | 39 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC017251 |
Gene symbol | ASGR2 |
Gene ID (NCBI) | 433 |
RRID | AB_2059814 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.
Protocols
Product Specific Protocols | |
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WB protocol for ASGR2 antibody 11501-2-AP | Download protocol |
IHC protocol for ASGR2 antibody 11501-2-AP | Download protocol |
IP protocol for ASGR2 antibody 11501-2-AP | Download protocol |
FC protocol for ASGR2 antibody 11501-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |