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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASH2L Monoclonal antibody

ASH2L Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G1H3

Cat no : 67859-1-Ig

Synonyms

Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASH2L Monoclonal antibody (67859-1-Ig)

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASH2L Monoclonal antibody (67859-1-Ig)

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ASH2L Monoclonal antibody (67859-1-Ig)

    IHC staining of human colon cancer using 67859-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67859-1-Ig (ASH2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using ASH2L Monoclonal antibody (67859-1-Ig)

    FC experiment of HeLa using 67859-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ASH2L (67859-1-Ig, Clone:1G1H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67859-1-Ig targets ASH2L in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ASH2L fusion protein Ag3008 相同性解析による交差性が予測される生物種
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC015936
Gene symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2918617
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASH2L antibody 67859-1-IgDownload protocol
IHC protocol for ASH2L antibody 67859-1-IgDownload protocol
FC protocol for ASH2L antibody 67859-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols