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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASK1 Monoclonal antibody, PBS Only

ASK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3A10

Cat no : 67072-1-PBS

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ASK1 Monoclonal antibody (67072-1-Ig)

    WB analysis of HEK-293 using 67072-1-Ig (same clone as 67072-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ASK1 Monoclonal antibody (67072-1-Ig)

    WB analysis of HSC-T6 using 67072-1-Ig (same clone as 67072-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ASK1 Monoclonal antibody (67072-1-Ig)

    WB analysis of NIH/3T3 using 67072-1-Ig (same clone as 67072-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ASK1 Monoclonal antibody (67072-1-Ig)

    WB analysis of MCF-7 using 67072-1-Ig (same clone as 67072-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ASK1 Monoclonal antibody (67072-1-Ig)

    WB analysis of Jurkat using 67072-1-Ig (same clone as 67072-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ASK1 Monoclonal antibody (67072-1-Ig)

    WB analysis of HeLa using 67072-1-Ig (same clone as 67072-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ASK1 Monoclonal antibody (67072-1-Ig)

    IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ASK1 Monoclonal antibody (67072-1-Ig)

    IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ASK1 Monoclonal antibody (67072-1-Ig)

    IHC staining of mouse heart using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ASK1 Monoclonal antibody (67072-1-Ig)

    IHC staining of mouse heart using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ASK1 Monoclonal antibody (67072-1-Ig)

    IHC staining of human lung cancer using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ASK1 Monoclonal antibody (67072-1-Ig)

    IHC staining of human lung cancer using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using ASK1 Monoclonal antibody (67072-1-Ig)

    IF Staining of RAW 264.7 using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ASK1 antibody (67072-1-Ig, Clone: 1C3A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67072-1-PBS targets ASK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASK1 fusion protein Ag5818 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated molecular weight 155 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC054503
Gene symbol ASK1
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.