Validation Data Gallery
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Tested Applications
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human retinoblastoma tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 15 publications below |
| WB | See 49 publications below |
| IHC | See 10 publications below |
| IF | See 2 publications below |
Product Information
14681-1-AP targets ASNS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ASNS fusion protein Ag6393 相同性解析による交差性が予測される生物種 |
| Full Name | asparagine synthetase |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 55-64 kDa |
| GenBank accession number | BC014621 |
| Gene Symbol | ASNS |
| Gene ID (NCBI) | 440 |
| RRID | AB_2060119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08243 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASNS antibody 14681-1-AP | Download protocol |
| IHC protocol for ASNS antibody 14681-1-AP | Download protocol |
| IF protocol for ASNS antibody 14681-1-AP | Download protocol |
| IP protocol for ASNS antibody 14681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase. | ||
Cell Metab Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.
| ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
Mol Cell Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
| ||
Nat Commun p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.
| ||
J Exp Med Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer. |