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  • KD/KO Validated

ASNS Polyclonal antibody

ASNS Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 14681-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

TS11, Glutamine-dependent asparagine synthetase, EC:6.3.5.4, Asparagine synthetase [glutamine-hydrolyzing]

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman retinoblastoma tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14681-1-AP targets ASNS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASNS fusion protein Ag6393 相同性解析による交差性が予測される生物種
Full Name asparagine synthetase
Calculated molecular weight 64 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC014621
Gene Symbol ASNS
Gene ID (NCBI) 440
RRIDAB_2060119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08243
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Protocols

Product Specific Protocols
WB protocol for ASNS antibody 14681-1-APDownload protocol
IHC protocol for ASNS antibody 14681-1-APDownload protocol
IF protocol for ASNS antibody 14681-1-APDownload protocol
IP protocol for ASNS antibody 14681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.

Authors - Robert T Williams
humanWB

Cell Metab

Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.

Authors - Abigail S Krall
  • KO Validated
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
  • KD Validated
humanWB, IHC

Mol Cell

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Authors - Ji Zhang
  • KD Validated
humanWB

Nat Commun

p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.

Authors - Longfei Deng
  • KD Validated
humanIHC

J Exp Med

Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.

Authors - Maria Victoria Recouvreux
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