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KD/KO Validated

ASPM Polyclonal antibody

Name: ASPM Polyclonal antibody
Brand: Proteintech
SKU: 26223-1-AP

ASPM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26223-1-AP

Synonyms

ASP, Asp homolog, ASPM, Calmbp1, MCPH5

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 26223-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26223-1-AP (ASPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 26223-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26223-1-AP (ASPM antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with alpha tubulin (66031-1-Ig, red).

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Cat No. 26223-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, K-562 cells
Positive IHC detected in	human prostate cancer tissue, human gliomas tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 8 publications below
IHC	See 7 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

The immunogen of 26223-1-AP is ASPM Fusion Protein expressed in E. coli.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ASPM fusion protein Ag24166 相同性解析による交差性が予測される生物種
Full Name	asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
Calculated molecular weight	410 kDa or 218 kDa
Observed molecular weight	218 kDa
GenBank accession number	BC034607
Gene symbol	ASPM
Gene ID (NCBI)	259266
RRID	AB_2880431
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ASPM antibody 26223-1-AP	Download protocol
IHC protocol for ASPM antibody 26223-1-AP	Download protocol
IF protocol for ASPM antibody 26223-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Aging (Albany NY) ASPM promotes glioblastoma growth by regulating G1 restriction point progression and Wnt-β-catenin signaling. Authors - Xin Chen KD Validated View Article
human	IHC	Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. Authors - Fangqiu Fu View Article
human	WB	Front Oncol Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis. Authors - Yingji Chen View Article
mouse	IHC	J Biol Chem The Transcription factor NF-YA is Crucial for Neural Progenitor Maintenance during Brain Development Authors - Tomoyuki Yamanaka View Article
human	WB	DNA Cell Biol ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth. Authors - Qian Yang View Article
human	WB,IHC	Int J Endocrinol ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway. Authors - Liang Jiang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASPM Polyclonal antibody

ASPM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 26223-1-AP

IHC staining of human prostate cancer using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human prostate cancer using 26223-1-AP

IHC staining of human prostate cancer using 26223-1-AP

IHC staining of human gliomas using 26223-1-AP

IF Staining of HeLa using 26223-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

ASPM promotes glioblastoma growth by regulating G1 restriction point progression and Wnt-β-catenin signaling.

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.

The Transcription factor NF-YA is Crucial for Neural Progenitor Maintenance during Brain Development

ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth.

ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway.