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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ASS1 Monoclonal antibody

ASS1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D1A11

Cat no : CL594-66036

Synonyms

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated ASS1 Monoclonal antibody (CL594-66036)

    IF Staining of MCF-7 using CL594-66036

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594-conjugated ASS1 antibody (CL594-66036, Clone: 1D1A11 ) at dilution of 1:100, 488phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated ASS1 Monoclonal antibody (CL594-66036)

    IF Staining of MCF-7 using CL594-66036

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL594-66036 (ASS1 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66036 is ASS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASS1 fusion protein Ag9314 相同性解析による交差性が予測される生物種
Full Name argininosuccinate synthetase 1
Calculated molecular weight 412 aa, 47 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC009243
Gene symbol ASS1
Gene ID (NCBI) 445
RRIDAB_2883485
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Protocols

Product Specific Protocols
IF protocol for CL594 ASS1 antibody CL594-66036Download protocol
Standard Protocols
Click here to view our Standard Protocols