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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASUN Recombinant antibody (84252-4-RR)

    WB analysis using 84252-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASUN Recombinant antibody (84252-4-RR)

    IF Staining of HeLa using 84252-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84252-4-RR

    Affinity and Kinetic Characterization of 84252-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84252-4-RR against Human ASUN were performed. The affinity constant is below 1 pM.

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ASUN Recombinant antibody

ASUN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84252-4-RR
Clone No.241586E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84252-4-RR targets ASUN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASUN fusion protein Ag13727 相同性解析による交差性が予測される生物種
Full Name chromosome 12 open reading frame 11
Calculated molecular weight 706 aa, 80 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC003081
Gene Symbol ASUN
Gene ID (NCBI) 55726
RRIDAB_3671801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NVM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

Protocols

Product Specific Protocols
WB protocol for ASUN antibody 84252-4-RRDownload protocol
IF protocol for ASUN antibody 84252-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols