• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASXL1 Polyclonal antibody

ASXL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16803-1-AP

Synonyms

ASXL1, KIAA0978

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ASXL1 Polyclonal antibody (16803-1-AP)

    WB analysis using 16803-1-AP

    Various lysates were subjected to Tris-acetate gel system followed by western blot with 16803-1-AP (ASXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

☆1/31（金）までお得に買える！ >> IHC・ELISA・IPキット最大30%OFFキャンペーン

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16803-1-AP targets ASXL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASXL1 fusion protein Ag10290 相同性解析による交差性が予測される生物種
Full Name additional sex combs like 1 (Drosophila)
Calculated molecular weight84aa,10 kDa; 1541aa,165 kDa
Observed molecular weight~200 kDa
GenBank accession numberBC064984
Gene symbol ASXL1
Gene ID (NCBI) 171023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Additional sex combs-like 1 (ASXL1) is a member of the ASXL family, which is involved in epigenetic regulation. It is one of the mammalian Asx homologs. Mutations in the ASXL1 gene are frequently found in myeloid neoplasms, breast cancers and prostate cancers (PMID: 30013160; PMID: 22436456; PMID: 26470845). In patients with myeloid malignancies, ASXL1 mutations are mostly heterozygous frameshift or nonsense mutations that result in C-terminal truncation (PMID: 30982744; PMID: 26095772; PMID: 32683582; PMID: 29643185).

Protocols

Product Specific Protocols
WB protocol for ASXL1 antibody 16803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols