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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATAD1 Polyclonal antibody

ATAD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16882-1-AP

Synonyms

hATAD1, FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATAD1 Polyclonal antibody (16882-1-AP)

    WB analysis using 16882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16882-1-AP (ATAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ATAD1 Polyclonal antibody (16882-1-AP)

    IP experiment of mouse brain using 16882-1-AP

    IP result of anti-ATAD1 (IP:16882-1-AP, 4ug; Detection:16882-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ATAD1 Polyclonal antibody (16882-1-AP)

    IHC staining of human colon cancer using 16882-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16882-1-AP (ATAD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16882-1-AP targets ATAD1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATAD1 fusion protein Ag10343 相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 1
Calculated molecular weight 361 aa, 41 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC010868
Gene symbol ATAD1
Gene ID (NCBI) 84896
RRIDAB_2878327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

Protocols

Product Specific Protocols
WB protocol for ATAD1 antibody 16882-1-APDownload protocol
IHC protocol for ATAD1 antibody 16882-1-APDownload protocol
IP protocol for ATAD1 antibody 16882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols