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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATAD1 Monoclonal antibody, PBS Only (Detector)

ATAD1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A2C3

Cat no : 60442-2-PBS

Synonyms

FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ATAD1 Monoclonal antibody (60442-2-Ig)

    WB analysis using 60442-2-Ig (same clone as 60442-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60442-2-Ig (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60442-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATAD1 Monoclonal antibody (60442-2-Ig)

    WB analysis using 60442-2-Ig (same clone as 60442-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60442-2-Ig (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60442-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50598-1

    Cytometric bead array standard curve of MP50598-1

    Cytometric bead array standard curve of MP50598-1, ATAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60442-1-PBS. Detection antibody: 60442-2-PBS. Standard:Ag10471. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60442-2-PBS targets ATAD1 as part of a matched antibody pair:

MP50598-1: 60442-1-PBS capture and 60442-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATAD1 fusion protein Ag10471 相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 1
Calculated molecular weight 361 aa, 41 kDa
Observed molecular weight32-37 kDa
GenBank accession numberBC010868
Gene symbol ATAD1
Gene ID (NCBI) 84896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.