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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATAD1 Recombinant antibody

ATAD1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241449D12

Cat no : 84194-6-RR

Synonyms

FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATAD1 Recombinant antibody (84194-6-RR)

    WB analysis using 84194-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84194-6-RR (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATAD1 Recombinant antibody (84194-6-RR)

    IF Staining of HeLa using 84194-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATAD1 antibody (84194-6-RR, Clone: 241449D12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using ATAD1 Recombinant antibody (84194-6-RR)

    FC experiment of A431 using 84194-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ATAD1 Recombinant antibody (84194-6-RR, Clone:241449D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84194-6-RR

    Affinity and Kinetic Characterization of 84194-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84194-6-RR against Human ATAD1 were performed. The affinity constant is 0.573 nM.

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Tested Applications

Positive WB detected inLNCaP cells, A549 cells, Jurkat cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84194-6-RR is ATAD1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATAD1 fusion protein Ag10343 相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 1
Calculated molecular weight 361 aa, 41 kDa
Observed molecular weight32-37 kDa
GenBank accession numberBC010868
Gene symbol ATAD1
Gene ID (NCBI) 84896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

Protocols

Product Specific Protocols
WB protocol for ATAD1 antibody 84194-6-RRDownload protocol
IF protocol for ATAD1 antibody 84194-6-RRDownload protocol
FC protocol for ATAD1 antibody 84194-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols