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  • KD/KO Validated

ATAD3A/B Polyclonal antibody

ATAD3A/B Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16610-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

AAA TOB3, ATAD3A, ATAD3B, KIAA1273, TOB3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inMAF cells
Positive IP detected inNCCIT cell
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16610-1-AP targets ATAD3A/B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATAD3A/B fusion protein Ag9836 相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 3B
Calculated molecular weight 72 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC002542
Gene Symbol ATAD3B
Gene ID (NCBI) 83858
RRIDAB_2878288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T9A4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties. This antibody can detect both ATAD3A and ATAD3B.

Protocols

Product Specific Protocols
IHC protocol for ATAD3A/B antibody 16610-1-APDownload protocol
IP protocol for ATAD3A/B antibody 16610-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
humanWB,CoIP

Nat Commun

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

Authors - Ayesha Sen
humanWB

EMBO J

ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.

Authors - Li Shu
  • KO Validated
ratWB

Oxid Med Cell Longev

miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.

Authors - Yandong Bao
  • KD Validated
mouseIF

Int J Mol Sci

Analysis of Radiation Toxicity in Mammalian Cells Stably Transduced with Mitochondrial Stat3

Authors - Alisa Zanin
mouseWB

J Cell Sci

ATAD3 controls mitochondrial cristae structure, influencing mtDNA replication and cholesterol levels in muscle.

Authors - Susana Peralta
  • KO Validated
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