Validation Data Gallery
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Tested Applications
| Positive WB detected in | MAF cells |
| Positive IP detected in | NCCIT cell |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 13 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
16610-1-AP targets ATAD3A/B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATAD3A/B fusion protein Ag9836 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase family, AAA domain containing 3B |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC002542 |
| Gene Symbol | ATAD3B |
| Gene ID (NCBI) | 83858 |
| RRID | AB_2878288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T9A4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties. This antibody can detect both ATAD3A and ATAD3B.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATAD3A/B antibody 16610-1-AP | Download protocol |
| IP protocol for ATAD3A/B antibody 16610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
EMBO J ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.
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Oxid Med Cell Longev miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.
| ||
Int J Mol Sci Analysis of Radiation Toxicity in Mammalian Cells Stably Transduced with Mitochondrial Stat3 | ||
J Cell Sci ATAD3 controls mitochondrial cristae structure, influencing mtDNA replication and cholesterol levels in muscle.
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