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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATAD4 / PRR15L Polyclonal antibody

ATAD4 / PRR15L Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25693-1-AP

Synonyms

ATAD4, Protein ATAD4, PRR15L

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATAD4 / PRR15L Polyclonal antibody (25693-1-AP)

    IHC staining of mouse kidney using 25693-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATAD4 / PRR15L Polyclonal antibody (25693-1-AP)

    IHC staining of mouse kidney using 25693-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATAD4 / PRR15L Polyclonal antibody (25693-1-AP)

    IF Staining of A431 using 25693-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATAD4 / PRR15L antibody (25693-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25693-1-AP targets ATAD4 / PRR15L in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATAD4 / PRR15L fusion protein Ag22500 相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 4
Calculated molecular weight 103 aa, 12 kDa
GenBank accession numberBC002865
Gene symbol ATAD4
Gene ID (NCBI) 79170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATAD4 (ATPase family AAA domain-containing protein 4), also known as PRR15L (Proline Rich 15 Like), and its function has not yet been discovered.

Protocols

Product Specific Protocols
IHC protocol for ATAD4 / PRR15L antibody 25693-1-APDownload protocol
IF protocol for ATAD4 / PRR15L antibody 25693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols