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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATF2 Polyclonal antibody (11908-1-AP)

    WB analysis using 11908-1-AP (same clone as 11908-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ATF2 Polyclonal antibody (11908-1-AP)

    WB analysis of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ATF2 Polyclonal antibody (11908-1-AP)

    IP experiment of mouse brain using 11908-1-AP (same clone as 11908-1-PBS)

    IP result of anti-ATF2 (IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATF2 Polyclonal antibody (11908-1-AP)

    IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11908-1-AP (ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ATF2 Polyclonal antibody (11908-1-AP)

    IF Staining of PC-3 using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATF2 Polyclonal antibody (11908-1-AP)

    IF Staining of HeLa using 11908-1-AP (same clone as 11908-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11908-1-PBS in a different storage buffer formulation.

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ATF2 Polyclonal antibody, PBS Only

ATF2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 11908-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11908-1-PBS targets ATF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF2 fusion protein Ag2581 相同性解析による交差性が予測される生物種
Full Name activating transcription factor 2
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 60 kDa, 64 kDa
GenBank accession numberBC026175
Gene Symbol ATF2
Gene ID (NCBI) 1386
RRIDAB_10695892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15336
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ).